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人毛囊外根鞘細胞 HHORSC

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產品名稱: 人毛囊外根鞘細胞 HHORSC
產品型號: HHORSC
產品展商: ScienCell
產品價格: 0.00 元
產品文檔: 無相關文檔

簡單介紹

人毛囊外根鞘細胞 HHORSC 人毛囊外根鞘細胞 HHORSC 上海 安徽 合肥 ScienCell細胞庫?


人毛囊外根鞘細胞 HHORSC  的詳細介紹
人毛囊外根鞘細胞位于毛球基部的乳頭周圍,屬于再生細胞范疇。它是毛發(fā)生長和毛發(fā)中黑色素轉運的主要來源。在毛發(fā)生長的活躍階段,基質中的細胞快速增長和分化導致毛發(fā)增長。生發(fā)基質細胞經歷被稱為角質化的過程,形成一層角蛋白以抑制毛發(fā)脫落。毛囊具有基質細胞的免疫特權。濾泡細胞培植研究表明生發(fā)基質細胞能通過植入毛乳頭細胞來誘導新毛囊形成。
人毛囊外根鞘細胞提取于人毛囊外根鞘,原代凍存。每管含有細胞數(shù)>5x10^5 cells/ml,此細胞通過纖維粘連蛋白和CD105免疫熒光染色驗證,經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。

推薦培養(yǎng)基:間充質干細胞培養(yǎng)基 (MSCM, Cat. #7501)
貨號 2420
產地 美國
縮寫 HHORSC
規(guī)格 5 x 10^5 /1ml
用途 科研
儲存 液氮
運輸 干冰
關鍵字 人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  人毛囊外根鞘細胞 HHORSC  
公司簡介                     
    合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國代理商,專業(yè)經營實驗室科研用原代細胞原代細胞專用培養(yǎng)基、原代細胞無血清培養(yǎng)基、干細胞、干細胞培養(yǎng)基、干細胞無血清培養(yǎng)基的研究和開發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務,對購買項目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項目準確到位,都有相關專業(yè)人士進行維護,確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務!
聯(lián)系方式

               何經理,合肥星肽生物科技有限公司,Tel:0551-63802898      400-8702-898,                                  

               E-mail:info@qingbio.com      http://m.27846.cn      QQ:514713116

1.) Panicker SP, Ganguly T, Consolo M, Price V, Mirmirani P, Honda K, Karnik P. (2012) "Sterol intermediates of cholesterol biosynthesis inhibit hair growth and trigger an innate immune response in cicatricial alopecia." PLoS One. 7: e38449.

2.) Karnik P, Tekeste Z, McCormick TS, Gilliam AC, Price VH, Cooper KD, Mirmirani P. (2009) "Hair follicle stem cell-specific PPARgamma deletion causes scarring alopecia." J Invest Dermatol. 129: 1243-57.

Question:

Hi, I'm interested on acquiring an Outer Root Sheath (ORS) cell line, however I can't understand why you say these cells have mesenchymal morphology and stain against CD105. These are markers of mesenchymal cells but Outer Root Sheath (ORS) cells are epithelial cells! They should not stain for markers of mesenchymal cells. The mesenchymal cells present in the hair follicle are dermal papilla and dermal sheath cells, not ORS cells! ORS are extremely different cells, they have to interact with mesenchymal cells, but they have a very different origin and function in the hair follicle. I want to buy a cell line that represents this. If you say your cell line stains for mesenchymal markers, your characterization of the cell line tells me that probably it is not a pure cell line and that you must certainly have a mixture of ORS cells and mesenchymal cells, like dermal sheath cells, since these mesenchymal cells surround ORS cells in the hair follicle That assumption is also present when you say the optimal medium is a mesenchymal stem cell medium. Most certainly a mesenchymal stem cell medium is not the optimal medium to culture epithelial cells. I need a pure cell line of ORS cell to compare to another epithelial population I have, otherwise I would be spending money in a wrong comparison because I would be using a cell line with a heterogeneous population of epithelial and mesenchymal cells. Please is there anyone that can give a clear answer to these questions? Someone directly involved in the isolation process of this cell line? It would be really important. I only have until next Tuesday to find and buy the cell line I need because after that the University financial department will prevent me from doing orders until next February. I would really appreciate your help. Best regards, Carla Abreu

Answers:

  • Posted by Manoj Sharma on Tuesday, November 10, 2015

    Hi Carla,

    These cells are not a cell line, they are a mixed population of primary cells isolated directly from the outer root sheath of the hair follicle and cryopreserved at passage 0. I have attached the product sheet for these cells which describes them in greater detail. Please let me know if you have additional questions about these cells after reviewing the product sheet.

    Thanks & Regards,
    Manoj Sharma

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