貨號	1800
產地	美國
縮寫	HA
規格	5 x 10^5 /1ml
用途	科研
儲存	液氮
運輸	干冰
關鍵字	人星形膠質細胞 HA   人星形膠質細胞 HA  人星形膠質細胞 HA   人星形膠質細胞 HA  人星形膠質細胞 HA   人星形膠質細胞 HA
公司簡介	合肥星肽生物科技有限公司作為專業的ScienCell中國代理商，專業經營實驗室科研用原代細胞、原代細胞專用培養基、原代細胞無血清培養基、干細胞、干細胞培養基、干細胞無血清培養基的研究和開發。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務，對購買項目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項目準確到位，都有相關專業人士進行維護,確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務！
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1、Question:

Which genes could represent good housekeeping genes for RT-qPCR analysis of these cells? Any suggestions?

Answers:

We recommend β-actin, GAPDH, LDHA, NONO, and PPIH to serve as housekeeping genes for astrocyte qRT-PCR. We also recommend using multiple housekeeping genes for data normalization. In addition, in a qRT-PCR assay, we recommend using Genomic DNA (gDNA) Control (GDC) to detect possible gDNA contamination, and Positive PCR Control (PPC) to test whether samples contain inhibitors or other factors that may negatively affect gene expression results. ScienCell offers GeneQuery? Human Astrocyte Cell Biology qPCR Array Kit for astrocyte qRT-PCR analysis.

2、Question:

WAre this cell lines？

Answers:

They are primary cells, not immortalized cell lines. thanks for your interest

3、Question:

What is the recommended maximum number of passages that the primary human astrocytes can be grown to without losing phenotype? Is there a recommended freezing protocol?

Answers:

Human Astrocytes can be subcultured for 4-5 passages. However, for primary cells, the markers such as GFAP may be lost during each passage, even though they are still astrocytes. Therefore, we recommend that customers use the cells at early passage without expanding or passaging too much. We do not recommend re-freezing the cells for future use. thanks for your interest